6829.1:
Digital holographic microscopy (DHM), a new technology for high resolution 3D imaging at the nano-scale: proof of principle for applications in biology and metrology
Abstract
Digital Holographic optical Microscopy (DHM) is a new imaging technology applied to optical microscopy.
The main topic of this project is to bring the proof of principle of this technology for different applications in science and industry. The innovation resides in processing digitally the holograms acquired with a solid state camera. 3D images of objects are obtained by reconstructing numerically the complex wave front emanating from them. The performances achieved with this technology are unique. Among the attractive features of DHM, we mention:
· an extreme vertical resolution of 0.6 nanometer,
· a very high acquisition rate (no scan, no mobile parts, limited only by video acquisition frequency),
· ease of use,
· observation in reflection and transmission geometry
· observation of surface topology, ultra-thin layers and submicrometric defects
and, more specifically in the field of biology:
· unrivalled degree of non-invasiveness, opening very attractive perspectives in life sciences for the
· 3D real time monitoring of physiological and pathological activity of cell and tissue culture.
· observation of molecule aggregates, layers, at the nanometric scale.
The aim of the present application for funding is to develop scientific and technological skills in DHM, in order to provide an innovative and powerful tool in nanotechnology.