5803.2:
On-chip flow cytometry
Abstract
The objective of this project is to study and develop an integrated flow cytometry device that combines microfluidic channels and integrated micro-electrodes. Flow cytometry allows rapid single-cell analysis of large number of cells by passing them (100 or more cells per second) one by one in a fluid stream through the path of a laser or electrodes. In contrast to conventional laser flow cytometry, our device performs cell characterization by electrical impedance spectroscopy for probing membrane and cytoplasm parameters without the need of specific labelling. Electrical fields are also used for cell sorting by dielectrophoretic forces.
The system consists of a micromachined glass or silicon chip on which are engraved microfluidic channels and deposited metal electrodes. The chip is mounted on a holder that provides external fluidic and electrical connections. The integrated flow cytometry device can be used in a number of applications ranging from clinical diagnostic to drug screening assays.
The Microsystems Division of LEISTER Process Technologies, Sarnen is specialized in microsystems technology. LEISTER is currently developing new devices and systems for biosciences applications. In the project, LEISTER is contributing to the chip manufacturing and commercial aspects. The objective is to produce and to market an integrated flow cytometry module that is based on disposable, single use, microchips with electrical detection and separation.
The Institute of Microsystems at EPFL, Lausanne, focuses its research on materials and technology aspects as well as on electrical detection principle and single cell manipulation in electrical fields.
The Institute of Pharmacology and Toxicology at University of Lausanne provides broad expertise in cell electrophysiology and will contribute to the assessment of impedance spectroscopy as a mean to measure cell membrane properties.